Preliminary study of the phytoconstituents of Pleurotus ostreatus grown in coffee pulp waste
DOI:
https://doi.org/10.21754/tecnia.v30i2.806Keywords:
Pleurotus ostreatus, sterol 5α,8α-endoperoxides, fatty acidsAbstract
The mushroom Pleurotus ostreatus also called oyster mushroom is widely cultivated for its nutritional value, as well as, for its different health benefits and for its values in remediation. Laboratory N° 11 of the Universidad Nacional de Ingeniería of Peru is cultivating P. ostreatus making use of residues of coffee pulp as a substrate. The present work describes the identification of the major phytoconstituents in the apolar fractions of the organic crude extrac of oyster mushroom. After the transesterification of a fraction rich in fatty acids, the palmitic acid methyl ester was identified as the majority and of a second fraction were identified of the steroids 5α,8α-epidioxi-24(R)-metilcolesta-6en-3β-ol and 5α,8α-epidioxi-22E-ergosta-6,22-dien-3β-ol. The compounds were identified by gas chromatography analysis coupled to a mass spectrometer and by nuclear magnetic resonance of hydrogen and carbon 13.
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References
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[2] E. M. Mohamed y F. A. Farghaly, “Bioactive Compounds of fresh and dried Pleurotus ostreatus Mushroom”, International Journal of Biotechnology for Wellness Industries, vol. 3, no. 1, pp. 3–14, 2014
[3] R. C. G. Corrêa, T. Brignari, A. Bracht, R. M. Peralta y I. C. F. R. Ferreira, “Biotechnological, nutritional and therapeutic uses of Pleurotus spp. (Oyster mushroom) related with its chemical composition: A review on the past decade findings”, Trends in Food Science & Technology, vol. 50, pp. 103–117, 2016
[4] H.E. El Fakharany, B.M. Haroun, T.B. Ng y El-R. M. Redwan, “Oyster mushroom laccase inhibits hepatitis C virus entry into peripheral blood cells and hepatoma cells”. Protein & Peptide Letter, vol. 17, no. 8, pp. 1031–1039, 2010
[5] K.T. Chu, L.X. Xia y T.B. Ng, “Pleurostrin, an antifungal peptide from the oyster mushroom”, Peptide, vol. 26, no. 11, pp. 2098–2103, 2005
[6] T. Satou, K. Kaneko, W. Li y K. Koike, “The toxin produced by Pleurotus ostreatus reduces the head size of nematodes”, Biological and Pharmaceutical Bulletin, vol. 31, no. 4, pp. 574–576, 2008
[7] K. Piska, K. Sułkowska-Ziaja y B. Muszyńska, “Edible mushroom Pleurotus ostreatus (oyster mushroom)- its dietary significance and biological activity”. Acta Scientiarum Polonorum Hortorum Cultus, vol. 16, no. 1, pp. 151–161, 2017
[8] A. M. Younis, F-S. Wu y H.H. El Shikh, “Antimicrobial activity of extracts of the oyster culinary medicinal mushroom Pleurotus ostreatus (higher Basidiomycetes) and identification of a new antimicrobial compound”. International Journal of Medicinal Mushrooms, vol. 17, no. 6, pp. 579–590, 2015
[9] Y. Yaoita, K. Amemiya, H. Ohnuma, K. Furumura, A. Masaki, T. Matsuki y M. Kikichi, “Sterol constituents from five edible Mushrooms”, Chemical and Pharmaceutical Bulletin, vol. 46, no. 6, pp. 944–950, 1998
[10] V. Chobot, L. Opletal, L. Jahodář, A. V. Patel, C. G. Dacke y G. Blunden, “Ergosta-4,6,8,22-tetraen-3-one from the edible fungus Pleurotus ostreatus (Oyster fungus)”, Phytochemistry, vol. 45, no. 8, pp. 1669–1671, 1997
[11] N. Rodríguez y C. Jaramillo, “Cultivo de hongos comestibles del género Pleurotus sobre residuos agrícolas de la zona cafetera”, Federación Nacional de Cafeteros de Colombia (CENICAFE), boletín técnico 27, oct. 2004.
[12] D.F. Gusmão, D.M. Estork, M.L.B. Paciencia, I.E.C. Díaz, S.A. Frana, I.B. Suffredini, A.D. Varella, R.N. Younes, L.F.L. Reis, E.F.S. Montero, y M.M. Bernardi. “Preliminary evaluation of the acute toxicity related to Abarema auriculata to mice and investigation of cytotoxicity of isolated flavonones”. PharmacologyOnline, vol. 1, pp. 113 – 127, 2013
[13] Normas analíticas do Instituto Adolf Lutz. São Paulo, SP, Brasil: IMESP, 1985.
[14] I. E. C. Díaz, E. G. Gonçalves, M. O. M. Marques y M. Yoshida, “Incidencia de ácidos grasos en especies de araceaes”, Revista de la Sociedad Química del Perú, vol. 77, no. 4, pp. 275–284, 2011
[15] Radulovic NS and Đorđević ND Steroids from poison hemlock (Conium maculatum L.): a GC–MS analysis. Journal of the Serbian Chemical Society 76, no. 11, pp.1471 – 1483, 2011.
[16] A. Gauvin, J. Smadja, M. Aknin, R. Faure y E-M. Gaydo, “Isolation of bioactive 5α,8α-epidioxy sterols from the marine sponge Luffariella cf. variabilis”, Canadian Journal of Chemistry, vol. 78, no. 7, pp. 986 – 992, 2000
[17] A. A. L. Gunatilaka, Y. Gopichand, F. J. Schmitz y C. Djerassi “Minor and trace sterol in marine invertebrate. 26. Isolation and structure elucidation of nine new 5α,8α-epidioxy sterols from four marine organisms”, The Journal of Organic Chemistry, vol. 46, no. 19, pp. 3860 – 3866, 1981
[18] M. A. Ponce, J. A. Ramirez, L. R. Galagovsky, E. G. Gros y R. Erra-Balsells, “A new look into the reaction between ergosterol and singlet oxygen in vitro”, Photochemical & Photobiological Sciences, vol. 1, pp. 749 – 756, 2002
[19] E. Moretto y R. Fett, Tecnologia de óleos e gorduras vegetais na indústria de alimentos. São Paulo, SP, Brasil: Varela Editora e livraria LTDA, 1998.
[20] M. Bu, B. B. Yang y L. Hu, “Natural bioactive sterol 5α,8α-endoperoxides as drug lead Compounds”, Medicinal Chemistry, vol. 40, no. 10, pp. 709 – 716, 2014
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Published
2020-11-27
How to Cite
[1]
I. E. Collantes Díaz, J. I. Nieto Juárez, Ángel D. Cuzcano Ruiz, and W. A. Reyes López, “Preliminary study of the phytoconstituents of Pleurotus ostreatus grown in coffee pulp waste”, TECNIA, vol. 30, no. 2, pp. 64–68, Nov. 2020.
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